Cryobank of microorganisms



strains on agar-agar
Culture of defrosted strains on Agar-Agar

rack of strains storage
Rack of storage of the preserved strains
 

Preservation of the strains

The strains preserved at the HélioBiotec platform :

  • Insertion mutants of Chlamydomonas reinhardtii created in the laboratory
  • Strains of C. reinhardtii expressions genes of interest upwards or downwards
  • Mutant strains of Synechocystis PCC6803
  • Various species of microalgae which come from the whole world (CCAP, UTEX, SAG, Duke Center, ...)

The conservation of the microalgae is done with a freezing in a cryoconservator at a temperature lower than 180°C in liquid nitrogen.

This mode of preservation was chosen to avoid the genetic drift which can occur following too numerous subculturing on agar-agar.



Example of recording of strains in LabCollector
 

Management of the strains with LabCollector

At the platform, the software LabCollector is used for the management of strains.
This software allows us, between other things, to manage two important steps in the preservation of the strains:

  • The traceability of the operations
  • The planning of the periodic controls of viability.

During the controls of viability, tests of no contamination are also made.