Lipid analytical techniques

lipid droplets
Lipid droplets observed with a fluorescence microscope

Automated deposit of the sample on the thin layer

Lipids separated by thin layer chromatography

Our platform is fully equipped for lipid analysis (storage and membrane lipids, lipid polyesters, surface waxes).

We do routine analysis of lipids extracted from microalgae and plants:

  • Identification and quantification of fatty acids, waxes and polyester monomers using gas chromatography coupled with mass spectrometry (GC-MS, Focus-DSQ2: with chemical ionization and electron impact) and gas chromatography with flame ionization detector (GC-FID, Trace)
  • Profiling and quantification of membrane and storage lipid classes using high performance thin layer chromatography (HP-TLC, Camag)
  • Analysis of lipid molecular species using liquid chromatography coupled to mass spectrometry (LC-MS/MS) (Planned for winter 2010)

Useful links:

The Lipid Librairy
The Cyberlipid Center
The Kansas Lipidomic Center
Lipid Maps