Laser microdissection

This platform is hosted and managed by the Laboratory of Developmental Plant Biology (LBDP, IBEB, CEA of Cadarache)



Laser dissecting microscope
Leica LMD6000 Laser Dissecting Microscope
 

Laser Capture Microdissection (LCM)

Our facility hosts a Leica LMD6000 Laser Dissecting Microscope (LCM). This microscope is equipped with a set of objectives ranging from 1.25X to 150X. It is coupled to a 355 nm dissecting laser (pulsed diode laser). With this set-up, users can cut fresh or fixed tissues with a high precision, and dispatch the samples automatically into separate tubes for further analysis.



laser microdissection
Example of laser microdissection : isolation of a root tip from a plantlet (Arabidopsis thaliana; Svistoonof 2007 Nature genetics)
 

Imaging

This microscope is also equipped for regular imaging, including bright field, phase contrast, DIC and fluorescence modes. The observation of fluorescent molecules such as DAPI, GFP, RFP is made possible by the presence of a light source and appropriate filter cubes, along with 2 cameras (Hitachi HV D20 and Leica DFC300FX).